A Convenient Plant-Based Detection System to Monitor Androgenic Compound in the Environment.
Identifieur interne : 000B79 ( Main/Exploration ); précédent : 000B78; suivant : 000B80A Convenient Plant-Based Detection System to Monitor Androgenic Compound in the Environment.
Auteurs : Dong-Gwan Kim [Corée du Sud] ; Ramin Bahmani [Corée du Sud] ; Jae-Heung Ko [Corée du Sud] ; Seongbin Hwang [Corée du Sud]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2019.
Abstract
Environmental androgen analogues act as endocrine disruptors, which inhibit the normal function of androgen in animals. In the present work, through the expression of a chimeric gene specified for the production of the anthocyanin in response to androgen DHT (dihydrotestosterone), we generated an indicator Arabidopsis that displays a red color in leaves in the presence of androgen compounds. This construct consists of a ligand-binding domain of the human androgen receptor gene and the poplar transcription factor gene PtrMYB119, which is involved in anthocyanin biosynthesis in poplar and Arabidopsis. The transgenic Arabidopsis XVA-PtrMYB119 displayed a red color in leaves in response to 10 ppm DHT, whereas it did not react in the presence of other androgenic compounds. The transcript level of PtrMYB119 peaked at day 13 of DHT exposure on agar media and then declined to its normal level at day 15. Expressions of anthocyanin biosynthesis genes including chalcone flavanone isomerase, chalcone synthase, flavanone 3-hydroxylase, dihydroflavonol 4-reductase, UFGT (UGT78D2), and anthocyanidin synthase were similar to that of PtrMYB119. It is assumed that this transgenic plant can be used by nonscientists for the detection of androgen DHT in the environment and samples such as food solution without any experimental procedures.
DOI: 10.3390/plants8080266
PubMed: 31387207
PubMed Central: PMC6724103
Affiliations:
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first="Ramin" last="Bahmani">Ramin Bahmani</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Ko, Jae Heung" sort="Ko, Jae Heung" uniqKey="Ko J" first="Jae-Heung" last="Ko">Jae-Heung Ko</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Kyeonggi-do 17104, Korea.</nlm:affiliation><country xml:lang="fr">Corée du 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Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Bahmani, Ramin" sort="Bahmani, Ramin" uniqKey="Bahmani R" first="Ramin" last="Bahmani">Ramin Bahmani</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Ko, Jae Heung" sort="Ko, Jae Heung" uniqKey="Ko J" first="Jae-Heung" last="Ko">Jae-Heung Ko</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Kyeonggi-do 17104, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant & Environmental New Resources, Kyung Hee University, Kyeonggi-do 17104</wicri:regionArea><wicri:noRegion>Kyeonggi-do 17104</wicri:noRegion></affiliation></author><author><name sortKey="Hwang, Seongbin" sort="Hwang, Seongbin" uniqKey="Hwang S" first="Seongbin" last="Hwang">Seongbin Hwang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea. sbhwang@sejong.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Plants (Basel, Switzerland)</title><idno type="ISSN">2223-7747</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Environmental androgen analogues act as endocrine disruptors, which inhibit the normal function of androgen in animals. In the present work, through the expression of a chimeric gene specified for the production of the anthocyanin in response to androgen DHT (dihydrotestosterone), we generated an indicator <i>Arabidopsis</i> that displays a red color in leaves in the presence of androgen compounds. This construct consists of a ligand-binding domain of the human androgen receptor gene and the poplar transcription factor gene <i>PtrMYB119</i>, which is involved in anthocyanin biosynthesis in poplar and <i>Arabidopsis</i>. The transgenic <i>Arabidopsis</i> XVA-<i>PtrMYB119</i> displayed a red color in leaves in response to 10 ppm DHT, whereas it did not react in the presence of other androgenic compounds. The transcript level of <i>PtrMYB119</i> peaked at day 13 of DHT exposure on agar media and then declined to its normal level at day 15. Expressions of anthocyanin biosynthesis genes including chalcone flavanone isomerase, chalcone synthase, flavanone 3-hydroxylase, dihydroflavonol 4-reductase, UFGT (UGT78D2), and anthocyanidin synthase were similar to that of <i>PtrMYB119</i>. It is assumed that this transgenic plant can be used by nonscientists for the detection of androgen DHT in the environment and samples such as food solution without any experimental procedures.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31387207</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>8</Issue><PubDate><Year>2019</Year><Month>Aug</Month><Day>05</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>A Convenient Plant-Based Detection System to Monitor Androgenic Compound in the Environment.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E266</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants8080266</ELocationID><Abstract><AbstractText>Environmental androgen analogues act as endocrine disruptors, which inhibit the normal function of androgen in animals. In the present work, through the expression of a chimeric gene specified for the production of the anthocyanin in response to androgen DHT (dihydrotestosterone), we generated an indicator <i>Arabidopsis</i> that displays a red color in leaves in the presence of androgen compounds. This construct consists of a ligand-binding domain of the human androgen receptor gene and the poplar transcription factor gene <i>PtrMYB119</i>, which is involved in anthocyanin biosynthesis in poplar and <i>Arabidopsis</i>. The transgenic <i>Arabidopsis</i> XVA-<i>PtrMYB119</i> displayed a red color in leaves in response to 10 ppm DHT, whereas it did not react in the presence of other androgenic compounds. The transcript level of <i>PtrMYB119</i> peaked at day 13 of DHT exposure on agar media and then declined to its normal level at day 15. Expressions of anthocyanin biosynthesis genes including chalcone flavanone isomerase, chalcone synthase, flavanone 3-hydroxylase, dihydroflavonol 4-reductase, UFGT (UGT78D2), and anthocyanidin synthase were similar to that of <i>PtrMYB119</i>. It is assumed that this transgenic plant can be used by nonscientists for the detection of androgen DHT in the environment and samples such as food solution without any experimental procedures.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Dong-Gwan</ForeName><Initials>DG</Initials><Identifier Source="ORCID">0000-0003-0092-5620</Identifier><AffiliationInfo><Affiliation>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bahmani</LastName><ForeName>Ramin</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ko</LastName><ForeName>Jae-Heung</ForeName><Initials>JH</Initials><Identifier Source="ORCID">0000-0002-4035-874X</Identifier><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Kyeonggi-do 17104, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hwang</LastName><ForeName>Seongbin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea. sbhwang@sejong.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>NRF-2015M3C8A6A06014500</GrantID><Agency>National Research Foundation of Korea</Agency><Country></Country></Grant><Grant><GrantID>IPET 316087-4</GrantID><Agency>Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>08</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arabidopsis thaliana</Keyword><Keyword MajorTopicYN="N">PtrMYB119</Keyword><Keyword MajorTopicYN="N">androgen receptor</Keyword><Keyword MajorTopicYN="N">anthocyanin</Keyword><Keyword MajorTopicYN="N">dihydrotestosterone</Keyword><Keyword MajorTopicYN="N">endocrine disruptor</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>07</Month><Day>15</Day></PubMedPubDate><PubMedPubDate 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